Effect of gamma irradiation on regeneration of mothbean

An experiment was carried out to find out the effect of gamma irradiation treatment to mothbean seeds on morphogenetic potential of callus derived from primary leaves of seedlings from treated seeds. A concomitant decrease in germination percentage was observed as the dose of gamma irradiation increased from 200 to 800 Gy. Regeneration potential of leaf callus derived from primary leaves of treated seeds was increased with lower doses of gamma irradiations (200 Gy and 400 Gy). The seeds irradiated at 400 Gy showed marked increase in number of shoot bud primordia, number of shoots/explant. Moreover, the plantlets regenerated from such explants produced more number of flowers under in vitro conditions. Genotype Jadia was found highly responsive to in vitro mutagenesis.