Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur 313 001, Rajasthan, India.
Present Address: *AICSIP, Potato Research Station, S.D. Agricultural University, DEESA, B.K. 385 535, Gujarat, India; Email: skjain_sdau@yahoo.co.in
Studies on effect of different mutagens were carried out with three concentrations of EMS (0.2, 0.4 and 0.6%) and DMS (0.02, 0.04 and 0.06%) along with growth regulators viz., indol acetic acid and gibberellic acid (3x10−3 M) on blackgram cultivar Dungla. A dose dependent reduction in germination, seedling height and seedling dry weight was observed with increasing concentrations of mutagens. The mutagenic effect was neutralized by the GA and IAA treatment and increased the germination, seedling height and seedling dry weight, respectively. Induced pollen sterility was also significant and dose dependent at all the concentrations of mutagens and improvement in the pollen fertility was observed with post treatment of IAA and GA. A wide spectrum of chlorophyll and macro mutations was observed in the progenies of M2 generation. The frequency and spectrum of chlorophyll and macro mutants increased with increasing concentrations of the mutagens. DMS induced a broader spectrum of chlorophyll and macro mutations in M2 generation as compared to EMS. In the present investigation, DMS was more effective and EMS was efficient mutagen. Though effectiveness and efficiency computed by different methods varied in quantum, their relative proportion were comparable. A single method of studying mutagenic effectiveness and efficiency should be appropriate.
Blackgram, Effectiveness, Mutagenic, Mutation, Variability
