1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana–141 004, India.
2Indian Council of Agricultural Research, Krishi Bhavan, New Delhi – 110 014
3International Crops Research Institute for the Semi-Arid Tropics, Patancheru-502 324 (AP), India.
4School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141 004, India e-mail: ruchipau@gmail.com
Ascochyta blight (AB) caused by Ascochyta rabiei L. is a devastating disease of chickpea world-wide. Resistant germplasm has been identified and an F2 mapping population of chickpea cultivars with contrasting disease reactions to Ascochyta rabiei L. was used to identify the markers linked to the disease using STMS and ICCM markers. STMS markers showed 40% polymorphism between the parents. Bulked segregant analysis (BSA) was performed to identify markers linked to the AB resistance gene. Of the 252 markers, only 10 were found to be possibly linked. MAPMAKER version 3.0 gave the linkage map of LG5 region of the chickpea genome spanning a length of 275.2 cM. The AB resistance gene was present on LG5 flanked by the markers TA 42 and TR 35, with a distance of 45.0 cM and 38.6 cM, respectively. It was concluded that there was synteny in the linkage groups 1, 3, 5 and 7.
Ascochyta rabiei, BSA, Chickpea, ICCM markers, STMS markers
