Cross species amplification of adzuki bean derived microsatellite markers and diversity analysis in blackgram (Vigna mungo L. Hepper)

An investigation was carried out on a collection of 32 blackgram genotypes involving 26 accessions of Vigna mungo var. silvestris, a wild progenitor and six cultivated genotypes of blackgram to study the genetic diversity using forty SSR markers derived from adzukibean. There was successful cross-species amplification of adzukibean derived SSR markers in blackgram. The number of alleles produced by different SSR markers ranged from 2 to 7 with an average of 2.75 alleles per marker and the allele sizes varied from 90 – 340 bp with a total of 110 alleles. The SSR markers derivedfrom adzukibean could be successfully used in the molecular studies of blackgram. Dissimilarity measures and clustering analyses were made using SSR data. Thirty two genotypes were grouped into four clusters in UPGMA analysis and into eight groups in neighbour – joining method. Genotype Vigna mungo var. silvestris/10 was placed separatelyfromrest of the genotypes inboth the analyses. Minor variations at molecular levels were observed among the Vigna mungo var. silvestris accessions in neighbour-joining method than the UPGMA analysis. The SSRmarkers in present study could differentiate cultivated species from wild progenitors, indicating high level of polymorphismat molecular level which can be further utilized for molecular studies in black gram, based on inter-subspecific crosses for development of linkage map as well as molecular tagging of many economically important traits.