1MDS, Department of Oral and Maxillofacial pathology, Saraswati Dental College and Hospital, Lucknow, India
2M.D.S Head of Department, Department of Oral and Maxillofacial pathology, Saraswati Dental College and Hospital, Lucknow, India
3M.D.S Associate Professor, Department of Oral and Maxillofacial pathology, Saraswati Dental College and Hospital, Lucknow, India
4M.Sc, PhD, Department of Genetics, Sanjay Gandhi Post Graduate Institute, Lucknow, India
*Address for Correspondence: Dr. Parul Khare, MDS, Assistant Professor, Department of Oral and Maxillofacial pathology, Maharana Pratap College of Dentistry and Research Center, Gwalior. C/o Mr. V B Sinha, MZ 6, Kutumb apartments, Phase I Balwant Nagar, Gwalior 474001, Madhya Pradesh, India. Ph no: 09302217691/09560815358/07514010195. Email– parulsinha02@gmail.com
Online published on 29 April, 2015.
This study was designed to analyze the quantity and quality of DNA extracted from saliva using phenol chloroform method and to evaluate the use of salivary DNA for amplification of genes and its comparison with blood DNA for the purpose of forensic individualization. Materials and method: Blood and saliva samples were collected from 20 volunteers and DNA was extracted using phenol chloroform method. Quantity and quality of DNA was analyzed by spectrophotometery and PCR was used for amplification of short tandem repeats (STR) {Amelogenin (AMG) and von Willebrand factor (vWF)} which were then matched with blood DNA. Results: Mean quantity of DNA obtained in saliva was 48.4 ± 8.2 μg/ml and in blood was 142.5± 45.9 μg/ml. Quality of DNA obtained was found to be lower for saliva than that for blood but was sufficient enough to amplify the AMG and vWF genes in 85% and 45% samples respectively. A positive matching with the blood samples was achieved in 94% and 100% of amplified samples for AMG and vWF respectively. Conclusion: DNA can be successfully isolated from salivary samples in optimal quantity for purpose of amplification of STRs and has a high to be used in forensics.
DNA, Short tandem repeats (STRs), Amelogenin (AMG), von Willebrand factor (vWF)
