1Assistant Professor, Department of Forensic Science, SGT University, Gurgaon, Haryana, India
2Professor, Department of Forensic Medicine & Science, SGT University, Gurgaon, Haryana, India
3Principal Scientific Officer and Head, Department of Biology & DNA Profiling, Central Forensic Science Laboratory, Central Bureau of Investigation, New Delhi, India
4Senior Scientific Officer, Department of Biology & DNA Profiling, Central Forensic Science Laboratory, Central Bureau of Investigation, New Delhi, India
5Professor, Department of Mathematics, SGT University, Gurgaon, Haryana, India
6Reader, Department of Audiology & Speech-Language Pathology, Amity University, Gurgaon, Haryana, India
*Corresponding author, Dr Leena Kumari, Department of Forensic Science, SGT University, Gurgaon, Haryana, India, Email: leena.bhardwaj2000@gmail.com
Online published on 23 March, 2021.
Determination of sex of an individual has a crucial role in personal identification. This study aims to determine the sex of an individual from burnt teeth samples exposed to a temperature of 250°C for 15 minutes and 45 minutes respectively through amplification of amelogenin marker. Independent sample t-test analysis of 60 tooth samples revealed that the mean quantity (in ìg/l) and quality of DNA were significantly reduced after heating for 15 minutes (M=9.71, SD=0.45) compared to freshly extracted tooth samples (M=29.91, SD=0.65), t(38)=114.19, p<0.000. The present study is valuable for human identification and the gender identification finally leading to individualization.
Amelogenin, DNA, Hydroxyapetite, Gender Identification, Electrophoresis, Forensic Investigation
