Optimization of process parameters for protease production by Bacillus licheniformis F1 in submerged fermentation using response surface methodology and its application as a laundry additive

A potential protease producing bacterial strain was isolated from mushroom compost and identified as Bacillus licheniformis F1 using 16S rRNA gene technique and deposited in NCBI gene bank vide accession number |KF769487|. The aim of this study was to enhance protease production by optimizing various process parameters through the statistical approach under submerged fermentation from this isolate. The optimum levels of these four parameters were determined employing the response surface central composite design (CCD). A maximum xylanase activity of 65.10 IU/ml had been obtained with 2% NH₄Cl, 9% tryptone, 0.2% CaCl₂ and 0.2% ZnSO₄.