1Department of Forensic Medicine, Sri Aurobindo Medical College and Pg Institute, Indore
2Sri Aurobindo Dental College and Pg Institute, Indore
Online published on 3 March, 2017.
Aspartic acid racemization has been found to be an accurate measure of age at death for recent forensic material. The aim of our study was to estimate the aspartic acid enantiomers ratio using HPLC-UV technique and Marfeys reagent as a derivatising agent from dentine samples of premolar teeth
Fifty individuals participated in this study in age groups of 11–60 yrs. Their teeth were processed for isolation of dentine, extraction of amino acid and analysis of aspartic acid enantiomers using HPLC and calculation of ratio of racemization.
The ratio of aspartic acid enantiomers showed increasing trend towards age of an individual.
The ratio of D/L aspartic acid was synchronized with age of an individual and it is appropriate method for age estimation.
Dentine, Aspartic acid, HPLC, Marfeys reagent, Age
