1Reader, Department of Oral Pathology & Microbiology, JSS Dental College and Hospital, Mysuru
2P G Student, Department of Oral Pathology & Microbiology, JSS Dental College and Hospital, Mysuru
3Reader, Department of Oral Pathology & Microbiology, JSS Dental College and Hospital, Mysuru
4Prof. & Head, Dept. of Microbilogy, JSS Medical College, Mysuru
*Corresponding Author: E-Mail: drvidyagd@gmail.com.
Online published on 11 October, 2017.
To evaluate the efficacy of various methods of sterilization on extracted human teeth.
100 non carious teeth were collected and sterilized using physical and chemical methods. Ten samples each were placed in seven different chemical disinfectants for a period of 7 days, at room temperature. The disinfectants used were 10% formalin, 3% hydrogen peroxide, 5.25% sodium hypochlorite, 70% alcohol, vinegar, thymol and Normal saline.10 teeth each were treated with three physical methods namely: microwave irradiation at 650 W for 3 min, boiling water at 1000C and autoclaving at 1210C for 20 minutes at 16 lbs pressure.
It was found that autoclaving at 121°C for 20 minutes at 16 lbs pressure, 5.25% sodium hypochlorite solution and10% formalin were 100% effective sterilization methods for extracted human teeth.
Autoclaving, 5.25% sodium hypochlorite and 10% formalin are the best methods for sterilization of extracted human teeth for research and preservation purposes.
Disinfection, Extracted Teeth, Preservation, Sterilization
