Journal of Indian Academy of Forensic Medicine
  • Year: 2018
  • Volume: 40
  • Issue: 2

UV Spectrophotometric Detection of Carbapenem Antibiotics in Forensic Samples

  • Author:
  • Singh Jaskaran1,, Upadhyaya Himanshi2, S.K. Shukla3, Sharma Madhulika4, Kataria Suraj5
  • Total Page Count: 5
  • Page Number: 179 to 183

1Assistant Professor, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab

2Assistant Professor, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab

3Prof. and Head, Amity Institute of Forensic Sciences, Amity University, Noida, U.P

4Former Director, State Forensic Science Laboratory, Govt. of NCT of Delhi

5PhD Student, Forensic Science Section Department of Anthropology, University of Delhi

*Corresponding Author: Email ID: Jaskaran.22220@lpu.co.in.

Online published on 4 December, 2018.

Abstract

In forensic samples, estimation of Carbapenem antibiotics has been done with the help of a simple, precise, cost effective U.V Spectrophotometric method, as described in the present study. Methanol was used as a solvent throughout the experiment. It was found that carbapenem antibiotics i.e., Meropenem, Doripenem, Imipenem and Etrapenem had 243, 211, 294, and 247 nm respectively, as absorption maxima. 2–20 microgram/ml was the range in which the Beer's law was obeyed. The recovery values found in forensic samples for carbapenem antibiotics were less than or equal to 70%. Hence, quantitative determination of carbapenem antibiotics in forensic samples was done by the proposed method.

Keywords

Carbapenem, Forensic Samples, U.V Spectrophotometer