In Vitro organogenesis of Trichosanthes dioica Roxb. Plantlets through callus culture

An efficient protocol for plantlet regeneration from shoot tip and axenic node derived callus was developed for Trichosanthes dioica Roxb. The optimum callus production was observed in MS + 2mg/l BAP + 4mg/l 2, 4-D, after 45 days of culture. Proliferation of multiple shoots (8) was obtained from the node derived callus cultures on MS + 1.5mg/l BAP + 0.3mg/l NAA, after 30 days of culture with an average shoot length of 4.88 ± 0.74cm. The elongated shoots rooted within seven to eight days in half-strength MS medium supplemented with 1.0mg/l IBA. The in vitro raised plantlets were sequentially acclimatized by transferring them in an inorganic MS salt solution, then to sterile distilled water and tap water before transferring to potted soil. The plantlets were successfully established in earthen pots filled with soil, sand and farmyard manure in a ratio of 1: 1: 1, showing 70% survival rate.