The Journal of Indian Botanical Society

UGC CARE (Group 1)
  • Year: 2010
  • Volume: 89
  • Issue: 1and2

Adenine Sulphate Enhanced In Vitro Shoot Regeneration in Centella Asiatic A (L.) Urban

  • Author:
  • Shalini Sharma, Y. Vimala
  • Total Page Count: 4
  • DOI:
  • Page Number: 30 to 33

Department of Botany, C.C.S. University, Meerut-2 50004, U.P.

Abstract

A protocol for multiple shoot regeneration in Centella asiatica, a medicinally valuable plant, is developed. Supplementation of MS medium with BAP (1.0 mg/l) and adenine sulphate (0.072 g/l) enhances shoot multiplication from leaf explants up to 11shoot buds per explant and 14–15 leaves per shoot with petiole length of about 7.0 cm as against 3.38 shoots per explant with 4.25 leaves per shoot reported so far. Increased number of leaves may enhance the content of bioactive metabolites especially asiaticoside to considerable extent reducing overexploitation of this valuable herb.

Keywords

adenine sulphate, Centella asiatica, shoot multiplication