In Vitro Regeneration in Emblica Officinalis From Juvenile Root-Derived Callus

Effect of NAA, BAP used singly and in combinnations were studied in seedling derived root explant of Emblica officinalis (Aonla) It was observed that BAP support shoot proliferation and multiplication. BAP used alone was found to be better than NAA alone or combination with BAP. Effect of subculturing was also observed in root-shoot junction explant of this important medicinal plant. It was noticed that with each progressive subculturing, percentage shoot proliferation increases. Within one week all explants produced callus. Differentiated micro shoots produced roots when inoculated on half strength MS media supplemented with 2.0 mg/l IBA.