1Krishi Vigyan Kendra, Hazipur, Bihar
Microbial & Molecular Genetics Laboratory, Department of Botany, Patna University, Patna-800005, India
*Corresponding Author, emial: bprasad.pu@gmail.com
Online published on 25 April, 2016.
A total of 102 strains of fungi isolated from soil samples in and around Patna. Three strains (AB50, AB55, AB109) showing amylase activity were screened for further investigation. Based on morpho-physiological characteristics the strain AB50 showing hyper-amylase activity was identified as Aspergillus fumigatus. The extra-cellular amylase production could be detected just after 24 hours under shaking condition and a complete hydrolysis of starch in the medium was observed after 96 hours incubation at 28+2°C. Partially purified amylase enzyme showed a maximum activity of 589U/100ml at pH 7.0 and 50°C temperature. This strain showed good growth in presence of Fe+2, Ca+2, Mg+2, Co2 up to a concentration of 5mM. Almost a two fold enhanced activity was observed in the presence of Fe2, Mg+2and Co2. However the ions like Hg+2, Mn+2 and Zn+2 remarkably decreased the activity of amylase.
Amylase production/activity, Aspergillus fumigatus, Metal ions
