Azadirachtaindica A. Juss. (Neem): Seed Pharmaceuticals Related to Growth Regulator Treatments

Endogenous titres of "neemrich ’was stimulated with exogenously applied hormones. In our efforts to study and control the commercial seed yield harvest, results distinctly indicated the regulatory influence of hormone combinations: kinetin-GA₃ and kinetin-spermidine in maintaining higher limonin levels during maturation relative to control samples. Variations in the amount of limonoid was observed during the five classes of growth regulator applications, i.e., putrescine, spermidine, spermine, GA₃ and kinetin. Limonoid content was generally high in samples treated with kinetin 0.2 mM and spermidine 0.2 mM. The levels decreased in 0.5 mM concentration treatments in spermine, putrescine and spermidine. Therefore, high concentrations of polyamines proved inhibitory to limonin levels. A steady rise in limonin was noted with kinetin while increasing the exogenous supply from 0.2 mM to 0.5 mM levels. Thus, the results are of potential pharmaceutical significance in terms of understanding and enhancing reproductive potential and seed yield.