In Vitro Shoot Proliferation in Barleria Prionitis Linn. From Nodal Explants

An efficient protocol for in vitro shoot proliferation of Barleria prionitis has been developed by using cotyledonary node as explant MS medium supplemented various concentrations of BAP (ranging from 0.2 mg/1 to 2mg/l) with all possible combinations of NAA and IAA separately in four different concentrations (0.1, 0.2, 0.5 and 1.0 mg/1) were used for selecting best suitable BAP-NAA and BAP-IAA combinations. Percentage of shoot proliferation increased from 0.2 to 0.5 mg/1 BAP and reached maximum (76% and 72%) at 1.0 mg/1 BAP with 0.1 mg/1 NAA and IAA, respectively. Higher concentrations of BAP (1.5 to 2.0 mg/1) as well as of auxins (0.2, 0.5 and 1.0 mg/1) suppressed shooting and induced callus formation.