Endogenous titres of “neemrich ’was stimulated with exogenously applied hormones. In our efforts to study and control the commercial seed yield harvest, results distinctly indicated the regulatory influence of hormone combinations: kinetin-GA3 and kinetin-spermidine in maintaining higher limonin levels during maturation relative to control samples. Variations in limonoids and seed oil was observed during the five classes of growth regulator applications, i.e., putrescine, spermidine, spermine, GA3 and kinetin. Limonoid content was generally high in samples treated with kinetin 0.2 mM and spermidine 0.2 mM. The levels of seed oil increased in 0.5 mM concentration treatments in spermine, putrescine and spermidine. The high concentrations of polyamines proved inhibitory to limonin levels and promotory to seed oil content. A steady rise in limonin was noted with GA3 while decreasing the exogenous supply from 0.5 mM to 0.2 mM levels. Thus, the results are of potential pharmaceutical significance in terms of understanding and enhancing regeneration and seed yield.
Azadirachta indica, GA3, kinetin, limonoids, neem, polyamines, seed oil
