Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad-211 002
Department of Botany, Government P.G. College, Lansdowne, Pauri Garhwal-246 139
*E-mail: drpandeybn@yahoo.co.in
Online published on 7 April, 2016.
To reduce the cost of micropropagation of banana, four experiments were conducted. In the first experiment, it was found that sucrose could be replaced by sugar cubes for multiplication and by even commercial grade sugar for rooting. In the second experiment, agar solidified medium was replaced by liquid medium supported with borosilicate glass beads in the rooting medium without affecting the 100% rooting. In the third experiment, IAA was removed from the rooting medium. The fourth experiment was conducted to replace the in vitro rooting by ex vitro rooting, but the results were poor. Cost of production has been further reduced by using the culture bottles of cheaper and re-usable soda-glass with polypropylene caps. The fast multiplication, perfect rooting and acclimatization further reduced the cost.
Banana, Ex-vitro rooting, Grande Naine, Low-cost, Micropropagation
