In vitro propagation studies of a threatened medicinal plant Boucerosia indica Wt. & Arn.
Abstract
The present work is intended to establish a reproducible protocol for rapid production of multiple shoots, callus cultures, rooting of in vitro regenerated shoots, hardening and transfer to the field conditions of a threatened medicinal plant Boucerosia indica. Nodal explants on Murashige and Skoog (MS) medium supplemented with combination of Benzy l6-aminopurine 2 mg/l and 2-Isopentyl adenine 1 mg/l showed very high percentage of shoot sprouting frequency. Internodal explants on MS medium with 2, 4-Dichlorophenoxy acetic acid 2.0 mg/l showed maximum response of callus proliferation. Subculture of above calli induced with Benzyl-6-aminopurine 2.0 mg/l was best for organogenesis. In vitro raised shoots rooted most excellent on = strength MS medium with Naphthalene-3-acetic acid 0.1 mg/l and those in vitro rooted plants showed 85% survival rate all through acclimatization.
Keywords
In vitro propagation, Boucerosia indica, Plant tissue culture, Explants, Regeneration
