Phytochemical investigations and micropropagation of Tylophora indica (Burm. F.) merill from nodal explants
Abstract
Tylophora indica (Burm. F.) Merill is commonly known as asthma herb. It is a threatened medicinal plant (climber) of the family Asclepiadaceae. The present paper deals with phytochemical studies in Tylophora indica. Plant extracts were analysed for phytochemical constituents. The qualitative and quantitative analysis confirmed the presence of various phytochemicals like Alkaloids, Flavonoids, Phenols, Saponins, Steroids, Tannins and Terpenoids. Some compounds were also estimated quantitatively (Alkaloids, Flavonoids and Saponins). In vitro clonal propagation is an alternative method of propagation. Multiple shoot buds were regenerated from nodal explants on MS medium supplemented with different concentrations and combinations of growth regulators and efficiently rooted on half-MS medium supplemented with IBA/IAA. The regenerated plantlets were successfully transferred to the glasshouse, acclimatized and transferred to the field.
Keywords
Axillary meristem, micropropagation, phytochemicals, qualitative analysis, shoot buds
