An Efficient Protocol for Micropropagation of A Medicinal Plant Aloe Vera L. Through Organ Culture

The aim of study was to assess the effect of different auxin and cytokinin on regeneration potential of rhizomatous stem (RSL and TRS) and leaf segment explants of Aloe vera and techniques were standardised for the mass propagation of this wonder drug plant. Sterilized explants (TRS, RSL, and LS) were cultured on the media supplemented with different combination of IAA, NAA, IBA, 2, 4-D, BAP and Kn within a range of 0.5–3.0mg/L. Best shoot proliferation (6–7 shoots/explants) was obtained on MS medium containing 2.5mg/L each of BAP and NAA from RSL explants and highest number of roots developed directly from LS explants in addition to callogenesis on MS medium fortified with NAA+IAA(2+1)mg/L. Optimum growth of callus was achieved from RSLand LS explants on medium containing NAA+BAP+IBA(2.5+2+0.5)mg/L. Callus in general was pale-yellow, hydrated and feathery in nature. Browning was a serious limitation in primary as well as callus culture which was minimised by ascorbic acid pre treatment. The best rooting of microshoots were obtained on shoot regenerating medium containing both BAP and NAA, 2.5mg/L each. In vitro grown plantlets were acclimatized and transferred to green house condition, regenerants were morphologically similar to mother plants.