Optimization Of in-Vitro Propagation Protocol for Plantago Ovata Forsk

The present investigation is an effort to establish callus formation and plant regeneration from cultured upper and lower hypocotyl explants of different varieties of Plantago ovata. Adventitious shoots were regenerated from cultured upper as well as lower hypocotyl explants excised from two to four weeks old in vitro grown seedlings of Plantago ovata on MS medium containing various concentration and combination of IAA, IBA, NAA, 2, 4-D, BAP and KN. Multiple shoot regeneration was observed from upper hypocotyl explants. Complete plant regeneration was observed from upper hypocotyl in MS media supplemented with low concentration (0.5 mg/L) of IBA, IAA and NAA separately and in combination of BAP (2.0 mg/L) and NAA (0.5 mg/L). When IAA (0.5, 1.5, 2.5, 5.0 mg/L), IBA (0.5, 1.5, 2.5, 5.0 mg/L) and NAA (0.5, 1.5, 2.5, 5.0 mg/L) were used separately in the media, there was remarkable growth of roots from both upper and lower hypocotyl parts. In lower concentration of IBA (0.5 mg/L) white and nodular callus growth with root formation was observed. 2, 4-D induced callusing in both the explants at uniform rate with the concentration ranging from 1.5 mg/L to 2.5 mg/L.