Isolation and Purification of Alkaloids from Achyranthes Aspera and Cissus Quadrangularis by HPLC

Methanolic extracts of Achyranthes aspera (seeds) and Cissus quadrangularis (stem) were evaluated for the presence of alkaloids by TLC. As the TLC showed positive results for the presence of alkaloids, further purification of total alkaloids was done using HPLC. HPLC profile of sample Achyranthes aspera revealed 2 major and 1 minor peaks with retention time 5.194 min, 5.567 min and 6.447 min respectively and their respective concentrations are 33.475 μg/ml, 29.963 μg/ml and 14.888 μg/ml respectively. HPLC profile of Cissus quadrangularis revealed 1 major and 3 minor peaks with retention time 5.549min and 6.036 min, 6.531 min, 7.655 min respectively and their respective concentrations are 38.417 μg/ml, 9.241 μg/ml, 18.412 μg/ml, 15.213 μg/ml (respectively. This study may give a scientific basis to plants already used for traditional purposes to treat various ailments and metabolic disorders which have not been evaluated.