Rapd-pcr evaluation of Carica papaya plants raised from the seeds treated with estrogen-A human sex hormone

RAPD-PCR evaluation of papaya (Carica papaya) plants raised from the seeds treated with Estrogen (Premarin) a human sex hormone was undertaken. Analysis of RAPD data of all the treatments showed significant genetic variation. Among 17 primers used, only two primers namely, OPF-12 and OPC-16 were properly amplified and had repeatable results. They were amplified template DNA to a total of 58 DNA fragments and showed polymorphism. The level of polymorphisms was different with different primers among different plants raised from 10 and 20 ppm premarin treated seeds. Cluster analysis based on UPGMA revealed two major clusters namely, Aand B with primer OPF-12 at a similarity coefficient of 64%. The cluster Ais the largest one with 10 genotypes and divided into two sub-clusters, namely, A₁ and A₂ at a similarity value of 76%. The sub-cluster A₁ again divides in two groups at similarity value 83%. In A_1(a) only 10 ppm premarin treated plants for 12 h and in A_1(b) control treated for 12 and 24 h were present. On the basis of present results, it is concluded that the plants raised from seeds treated with premarin human sex hormone have nearly same genotype.