A protocol for In vitro somatic embryogenesis from leaf explants of Andrographis paniculata wall. ex nees

Andrographis paniculata Wall. Ex Nees is a small branched annual medicinal herb. In vitro study was conducted for somatic embryogenesis through in vitro leaf explant on MS medium containing different doses of 2,4-D and BA. 4.44 μM BA with the combination of 0.45 μM 2,4-D produced maximum somatic embryos with in 60, i.e. 30+30 (intermittent one mid transfer on the same fresh medium) days after inoculation of trimmed leaf explants. Different strength of MS medium (1.0×, 0.75×, 0.50× and 0.25×) tested on somatic embryos for the maturation of embryos. 30 days after the inoculation of somatic embryos, 0.50 x gave 96% mature embryos. After that theses mature embryos developed into plants on MS medium containing 5 μM GA3 and 10 μM BA in 30 days after the inoculation of mature embryos. The somatic embryogenesis raised plantlets acclimatized and successfully transferred to the field and successfully survived.