Evaluation of the comparative efficacy of recombinant protein antigens based enzyme-linked immunosorbent assay for the diagnosis of caprine leptospirosis

A comparative study was done between LipL32 and LipL41 recombinant antigens based enzyme-linked immunosorbent assay (ELISA) with respect to the standard reference test-Microscopic Agglutination Test (MAT), using caprine serum samples. MAT was conducted using 11 Leptospira serovars. The LipL32 and LipL41 genes were amplified using specific primers and the polymerase chain reaction products (PCR) were then cloned and expressed. The optimum antigen concentration of 100 ng of each antigen was used for the screening of serum samples from goat suspected of leptospirosis. It was found that LipL32 recombinant antigen based ELISA detected more positive cases of illness than MAT.