Journal of Immunology and Immunopathology

In the present study, blood samples from Brucella melitensis Rev. 1 vaccinated and non-vaccinated (control) sheep were collected at definite interval from 0 day to 98^th day post vaccination (PV), sera were separated and subjected to different serological tests viz., Standard tube agglutination test (STAT), Rose Bengal Plate test (RBPT), Agar gel immunodiffision (AGID), Radial Immunodiffusion (RID), Enzyme-linked immunosorbent assay (ELISA), and dot-ELISA. The STAT titre of 40 IU and formation of precipitation line in AGID were found from 3^rd day to 84^th day post-vaccination (PV) whereas the characteristic precipitation ring appeared from 7^th to 84^th day PV in RID. The RBPT, ELISA and dot-ELISA were found positive from 3^rd to 98^th day PV, which revealed higher sensitivity of these tests in comparison to STAT, AGID and RID. However, RBPT, being a sensitive, simple, economic, rapid and field oriented test, was advised to be used for detection of antibodies against the brucellosis in sheep.