Journal of Immunology and Immunopathology

  • Year: 2009
  • Volume: 11
  • Issue: 2

Antigenic characteristics of cell-culture adapted inclusion body hepatitis virus of poultry

  • Author:
  • S K Saxena1,, R Chandra2, P Singh
  • Total Page Count: 4
  • DOI:
  • Page Number: 48 to 51

1Departement of MBGE, College of Basic Sciences and Humanities.

2Departement of Veterinary Microbiology, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, 263145, (Uttrakhand).

U P Pt. Deen Dayal Upadhyaya Pashu-Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, 281 001, (Uttar Pradesh), INDIA.

*Corresponding Author: E-mail: drsubodhsaxena@gmail.com

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Abstract

Two fowl adenovirus (FAV) isolates (IBH B and IBH B1) collected from outbreaks of private poultry farms in Bazpur area of Uttarakhand in year 2004 and 2005, respectively, were adapted in Chicken Embryo Liver (CEL) cell culture. The cell culture adapted virus grown in bulk was purified using sucrose gradient. The polypeptide pattern of purified virus was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A total of 9 polypeptides ranging in molecular weight between 35.2 and 110.0 kDa were observed. Western blot analysis of structural polypeptides also revealed that all 9 polypeptides were immunogenic. Out of these 9 polypeptides, 2 viz. 97.4 kDa and 53.9 kDa were found to be major immunogenic polypeptide based on intense staining.

Keywords

Inclusion Body Hepatitis, Immunogenic polypeptides, Structural polypeptides