Systemic immune response to avridine adjuvanted lentogenic Newcastle disease vaccine virus administered through intranasal route

In order to evaluate the adjuvant activity of avridine, a lipoidal amine compound, vaccines were prepared by co-administering with lentogenic Newcastle disease ‘F’ strain (NDF) through intranasal route in one week (wk) old broiler chicks. Serum samples were collected at weekly intervals upto 7 weeks post vaccination (PV) and antibody levels determined by haemagglutination inhibition (HI) and Enzyme linked immunosorbent assay (ELISA). The cellular immune responses were measured by in-vitro lymphocyte proliferation assay at 3 week and 7 week PV. The results showed that co-administration of avridine with NDF vaccine delivered through intranasal route influenced serum antibody levels neither positively nor negatively. The serum antibody levels were of similar levels compared to birds vaccinated with NDF vaccine alone. The proliferative responses of the peripheral blood mononuclear cells were also of similar levels to the NDF vaccine controls.