Journal of Immunology and Immunopathology

UGC CARE (Group 1)
  • Year: 2010
  • Volume: 12
  • Issue: 1

Characterization of surface lipoprotein P48 in Indian isolates of Mycoplasma agalactiae

  • Author:
  • S Kathiresan, V P Singh, P S Cheema, K Ltu, S Das
  • Total Page Count: 5
  • DOI:
  • Page Number: 64 to 68

Division of Bacteriology and Mycology, Indian Veterinary Research Institute, National Referral Laboratory on Mycoplasma, Izatnagar, 243122, (Uttar Pradesh), INDIA.Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, 243122, (Uttar Pradesh), INDIA.

Abstract

The surface lipoprotein P48 of Mycoplasma agalactiae has been reported to play an important role in immune response of infected animals and has also been designated as one of the most potent candidates for the diagnosis of contagious agalactia. The present investigation was carried out to study the presence of this lipoprotein in Indian isolates of M. agalactiae. SDS-PAGE analysis of the four Indian isolates along with the standard strain of M. agalactiae revealed a major band of 48 kDa. Subsequently, immunolblot assay using polyclonal serum against standard strain of M. agalactiae showed that 48 kDa protein is the major immunodominant protein. Further, immunoblot analysis with monospecific serum against recombinant P48 confirmed the presence of P48 in all the Indian isolates.

Keywords

Mycoplasma agalactiae, M. bovis, SDS-PAGE, western blot, lipoprotein