** Corresponding author: E-mail: pawanjitcheema@gmail.com.
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The surface lipoprotein P48 of Mycoplasma agalactiae has been reported to play an important role in immune response of infected animals and has also been designated as one of the most potent candidates for the diagnosis of contagious agalactia. The present investigation was carried out to study the presence of this lipoprotein in Indian isolates of M. agalactiae. SDS-PAGE analysis of the four Indian isolates along with the standard strain of M. agalactiae revealed a major band of 48 kDa. Subsequently, immunolblot assay using polyclonal serum against standard strain of M. agalactiae showed that 48 kDa protein is the major immunodominant protein. Further, immunoblot analysis with monospecific serum against recombinant P48 confirmed the presence of P48 in all the Indian isolates.
Mycoplasma agalactiae, M. bovis, SDS-PAGE, western blot, lipoprotein