Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, M.P. Email jitu_micro@hotmail.com.
CPV is a single stranded DNA virus and is a major pathogen of dogs. The virus is known to cause myocarditis in young puppies and hemorrhagic gastroenteritis in older animals. Over a period of time enteric form of disease has predominated and it persists as a major problem in breeding kennels, or where vaccination is widely practiced. Polymerase chain reaction was performed using reported primers for detection of Canine Parvo virus (CPV) in the stool sample obtained from repository. The PCR primers were specific to VP1/VP2 gene of CPV. Sensitivity assay of PCR detection was performed by making dilutions of CPV positive DNA sample and running each dilution in a PCR and visualising amplicons in ethidium bromide stained agarose gel under UV radiation. Study was valuable in determining the efficiency of PCR therefore is found to be of immense applicability in diagnosing virus in apparently healthy animals in latent stage or very early stage long before it shows any symptoms of disease in them thus controlling infection and formulating policies on prevention and control of disease at a very early stage at right time.
