1Haryana Veterinary Surgeon-I, Department of Animal Husbandry and Dairying, Haryana, INDIA
2Professor, Department of Veterinary Medicine, College of Veterinary Sciences
3Professor and Scientist-in-Charge, Veterinary College Central Laboratory, College of Veterinary Sciences
4Professor and Scientist-in-Charge, Regional Research Centre on FMD, Department of Veterinary Microbiology, College of Veterinary Sciences, LLRUVAS, Hisar- 125 004 (Haryana), INDIA
5Haryana Veterinary Surgeon-I, Frozen Semen Station, HLDB, Jagadhri-135003, Yamuna Nagar (Haryana), INDIA
The T-cell subpopulation (BoCD4+, BoCD8+ and B0WC1+ T cells) were analysed in milk samples collected from five normal and mastitic buffaloes by flow cytometry. Milk obtained from mastitic buffaloes revealed a significant increase in BoCD4+ and BoWC1+ T cells as compared with that from normal buffaloes (P<0.05). An increase was also recorded in BoCD8+ cells of mastitic buffaloes as compared with normal buffaloes, but this elevation was not significant (P>0.05).The mean ratio of BoCD4+: BoCD8+T lymphocytes in the milk of healthy animals was 0.9+0.03. However, this ratio was lowered in buffaloes with subclinical mastitis, indicating the presence of a higher proportion of BoCD8+ than of BoCD4+ T lymphocytes in their milk. Hence, this study showed that the CD4:CD8 ratio could be used as an indicator of susceptibility to bovine mastitis.
Mastitis, T cells, Phenotypic Alterations
