1Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
2Animal Biotechnology Centre, Gobind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
3Department of Veterinary Biochemistry, Assam Agriculture University, Khanapara, Guwahati, Assam, India
4Department of Veterinary Microbiology, Gobind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
*Email id: ak.hau.abt@gmail.com
Online published on 29 July, 2013.
Fowl typhoid caused by Salmonella enterica serovar Gallinarum remains to be a major problem for poultry health worldwide. The emergence of multidrug-resistant strains of Salmonella with increased virulence and survivability, leading to increased morbidity and mortality, has further complicated its management. Presently available vaccines against fowl typhoid have less-than-desired efficacy and unacceptable side effects, making it pertinent to search for new immunogens suitable for vaccine formulation. The outer membrane proteins (OMPs) of Salmonella have been considered as possible candidates for protection against typhoid. The present study was conducted to characterise the OMPs of Salmonella enterica serovar Gallinarum using immunodiffusion and western blot techniques. Presence of three precipitation lines against OMP preparation in immunodiffusion test indicated the presence of at least three immunogenic OMPs in S. Gallinarum. Further, western blot analysis revealed OMP 40 as one of the most immunopotent antigen. This study suggests that OMP 40 may be the suitable target for the development of vaccine formulation against fowl typhoid.
Fowl typhoid, Salmonella Gallinarum, OMPs, Vaccine, Western blotting
