Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, Bareilly
Online published on 19 December, 2013.
Hemorrhagic septicemia, caused by Pasteurella multocida (P.multocida) serotype B:2 is an economically important disease of cattle and buffaloes and causes serious economic losses to cattle industry in Asian subcontinent. A DNA vaccine encoding P. multocida Outer membrane protein(OmpH) gene (p V ompH) was constructed and its immune response and protective efficacy was evaluated in mice model. High titres of specific antibody and strong T cell proliferative response were observed in mice vaccinated with p V ompH (P<0.05), as compared with empty vector and PBS control. Furthermore, DNA vaccine induced balanced Th1 and Th2 response as detrmined by IgG isotype analysis. Despite strong humoral and cell mediated immune response DNA vaccine could only provide partial protection to mice against direct challenge studies with Live P.multocida. But survival time of mice in pVompH was longer than those in control groups.
