Institute of Biotechnology, G B Pant University of Agriculture & Technology, Patwadangar-263128, Distt. Nainital, Uttarakhand, India
Online published on 19 December, 2013.
Tinospora cordifolia is a common medicinal plant used in traditional medicine of India. Traditionally, the plant is being used for the treatment of various diseases. Phytochemical analysis of plant revealed the presence of alkaloids, flavonoids, steroids, saponins, total phenols, cardiac glycosides and reducing sugars. Therefore, it had been planned to study its anticancer properties in BHK-21 cells. Methanolic, ethanolic and aqueous extracts of the leaves of Tinospora cordifolia were screened for their anticancer properties. The cells were seeded with all the extracts and then allowed to grow for 24hrs; the cell growth was inhibited within 24hrs. The cytopathology included rounding and clumping of cells, detachment of cells, flagging of cells and apoptosis. Methanolic and ethanolic extracts showed better response than that of its methanolic extract. The concentration of 10 mg/ml of methanolic extract inhibited the cell growth with high affinity.
