Study on Bacterial Etiology of Skin and Wound Infections and Antibiogram Profile in Animals of Mathura Region in U.P.

A study on bacterial etiology of skin affections/wounds and on antibiogram profile was carried out in different species of animals of various age groups from Sept, 2012 to Nov, 2013. In this context, a total of 183 samples from cattle, buffalo, dogs, goats, sheep, camel, monkey and horses were collected from Mathura and nearby surrounding areas, processed and interpreted for bacterial etiological agent in the Department of Microbiology, DUVASU, Mathura. Clinical samples were collected using sterile swabs from wounds (horn abscess, gangrenous tail, chronic abscess, deep suppurative wounds due to injuries, open, gun-shot, lacerated, abrasive, incised, ulcerated and post-operative wounds) of various types. The organisms isolated were Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, E.coli, Klebsiella spp., Proteus spp, Clostridium spp and Bacillus spp. Bacterial isolates obtained were subjected to in vitro antibiotic sensitivity testing against 18 antibiotic discs by disc diffusion method of Kirby-Bauer method using Muller-Hinton agar. Results revealed maximum sensitivity to Gatifloxacin, Sparfloxacin, Gentamicin along with emergence of many multi-drug resistant bacterial strains. Isolation of multidrug resistant bacterial strains of Staphylococcus aureus, Pseudomonas aeruginosa and E.coli is an important finding in view of indiscriminate use of antibiotics. Developing multiple drug resistance patterns of Methicillin resistant Staphylococcus aureus is a matter of public health concern. Therefore, use of antimicrobial sensitivity testing is advised to know antibiogram of a particular causative agent to check the development of antibiotic resistance by prescribing most appropriate antibiotic therapy.