1Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, University of Animal and Fishery Sciences, 37, K.B. Sarani, PO. Belgachia, Kolkata-700037, West Bengal
*Corresponding author email id: dranjanmondal@gmail.com
Picobirnaviruses (PBVs) have recently been classified into the Picobirnaviridae family. They are small, non-enveloped viruses with bi-segmented, double-stranded (ds) RNA genomes. Identification of PBVs has been done using RNA-PAGE and reverse transcription PCR (RT-PCR). In this study, two (2.25%) calves were found positive for large genome profile picobirnavirus. Both RNA-PAGE positive picobirnavirus samples showed amplicons of approximate 201 bp using specific primer pair viz. PicoB25 (+) and PicoB43(-) for genogroup I picobirnavirus by RT-PCR.
Picobirnavirus, Genogroup I, RNA-PAGE, RT-PCR
