Journal of Immunology and Immunopathology
  • Year: 2014
  • Volume: 16
  • Issue: 1and2

Prevalence of Group A Rotaviruses (RVA) in Pig Population of North East India

  • Author:
  • DP Bora1,*, M Bora2, B Borah1, B Bezborah1, DK Sarma1, YPS Malik3, TK Dutta4
  • Total Page Count: 5
  • Published Online: Jan 1, 2014
  • Page Number: 53 to 57

1Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara campus, Guwahati-781022, Assam, India

2Division of Virology, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar PradeshIndia

3Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India

4Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, CAU, Selesih, Aizwal-796014, Mizoram, India

*Corresponding author email id: drdpbora@gmail.com

Abstract

Group A rotaviruses (RVA) have been recognised as an important cause of piglet diarrhoea which is a major threat causing serious economic losses in swine industry of north east India. Apart from RVA, there are many other agents which are directly or indirectly associated with piglet diarrhoea. Thus accurate and timely diagnosis is very much essential to establish the etiology of piglet diarrhoea. In the present study, RVA was detected in faecal samples of diarrhoeic pigs using RNA-PAGE and RT-PCR, collected from different states of Northeast India. Overall, of 601 faecal samples collected, 134(22.29%) samples were found to be positive for presence of RVA by RNA-PAGE. In RT-PCR, RVA was detected by amplification of VP4 and VP7 genes of RV, where 178 (29.61%) samples were found positive. Among the states, Assam and Tripura recorded highest prevalence of RV among pig population in comparison to other states of NER.

Keywords

Diarrhoea, Piglet, GARV, VP4 gene, VP7 gene, PCR