1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad-224229, Uttar Pradesh, India
2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad-224229, Uttar Pradesh, India
3Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad-224229, Uttar Pradesh, India
*Corresponding author email id: namitajoshivet@gmail.com
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study, 44 samples consisting of faeces (31) and intestinal tissue (13) collected from dogs clinically suffering from CPV were subjected to plate and slide haemagglutination (HA) test using pig RBCs and were processed for isolation of the virus in Crandell Rees feline kidney (CRFK) cells. Of these, 25 faecal and nonintestinal tissue samples were found positive for CPV antigen using plate and slide HA test. The growth of virus in cell culture was adjudged by cytopathic effects, and its presence was confirmed by determining HA and infectivity titres in cell culture supernatants. The infected cells showed intense cellular rounding and clumping. The HA titres and infectivity titres in cell culture supernatants increased gradually from 8 to 1024 and 2 × 106.7 to 1012.3 TCID50/ml at 2nd and 5th passage, respectively.
Canine parvovirus, CRFK cell, Cytopathic effects, Haemagglutination test, Infectivity titres, May-Grunwald Giemsa staining, Fluorescent antibody technique
