1Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
2Immunology Section, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
3Biological product, CADRAD, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
4Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
5Unidad de Sanidad y Biotecnología, Instituto de Investigaciónen Recursos Cinegéticos, Universidadde Castilla-La Mancha, Ciudad Real, Spain
*Corresponding author email id: drmithileshsingh@yahoo.com
Objectives: Isolation of Canine parvovirus -2 in A-72 cell line. Material and method: In the present study, out of 20 clinical samples (faecal swab), five samples were subjected to CPV-2 isolation in A-72 cell linebased on positive confirmation in both polymerase chain reaction (PCR) and haemagglutination (HA) test. Result: Out of five confirmed PCR and HA positive faecal sample only in one A-72 cell line flask cytopathic effect was seen in mild form. Discussion: A-72 cell line permit the faster growth of CPV-2 than other cell line as pronounced CPE was seen. However four PCR and HA positive faecal samples were failed to grow in A-72 cell line might be due to lack of sufficient virus titre as 8–10 days of post-infection specific anti-CPV antibodies formed in the intestinal lumen frequently entrap many of the virions thus preventing the virus adsorption to cells. Conclusion:Although CPV-2 was isolated in one of the infected A-72 cell line flask but visual confirmation by CPE was poor. Therefore, it is recommended that Fluorescent antibody test (FAT) is only way for proper visualisation of CPV-2 induced CPE in any suitable cell line
CPV-2, HA, BBS, PCR, FAT, DNA, PBS
