Journal of Immunology and Immunopathology
  • Year: 2017
  • Volume: 19
  • Issue: 1

Pathomorphological and Molecular Investigation of Cutaneous Papillomatosis in a Saint Bernard Dog

  • Author:
  • Adya Prakash Rath1, Renu Singh2, Upendra P. Lambe3, Abhilash Routray4, Krutanjali Swain5, Saraswat Sahoo6, Subha Ganguly7,*
  • Total Page Count: 5
  • Published Online: Jan 1, 2017
  • Page Number: 31 to 35

1National Research Centre on Equines, Hisar, Haryana, India

2Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

3Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

4Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

5Department of Veterinary Parasitology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

6Department of Animal Reproduction Gynaecology & Obstetrics, Orissa University of Agriculture and Technology, Bhubaneswar, Orissa, India

7Department of Veterinary Microbiology, Arawali Veterinary College (Affiliated with Rajasthan University of Veterinary and Animal Sciences, Bikaner), N.H.-52 Jaipur Road, V.P.O Bajor, Dist. Sikar, Rajasthan

*Corresponding author e-mail id: ganguly38@gmail.com

Abstract

Papilloma is a benign exophytic neoplastic proliferation of the squamous epithelium caused by infection with papillomavirus. This report describes a case of cutaneous papillomatosis in a 6-year-old male Saint Bernard dog, which was presented with clinical signs of cauliflower like outgrowth over the neck region. Materials and Methods: Grossly, the surface was having a tuft of nodules with horn-like projections from skin. Cut sections consisted of multiple fronds with keratinous material accumulating on the surface. Results: Histopathologic examination revealed outward papillary projections of stratified squamous epithelium having keratinised layer on the periphery and connective tissue in the centre. Papanicolaou stain revealed orange-coloured keratinised layer. Blood and tissue samples were analysed for Canine Papillomavirus 1 (CPV1) by polymerase chain reaction (PCR) using specific primers for PVL1 gene. Conclusion: The PCR amplification of CPV amplified a 480-bp fragment of the PVL1 gene.

Keywords

Exophytic growth, Papillomavirus, Field’s stain, Keratinised epithelium, L1 gene