Ideal Sample for Isolation of Pasteurella multocida and Susceptibility of Field Isolates Against Commonly Used Antibiotics

Haemorrhagic septicaemia (HS) is an acute pasteurellosis manifested by an acute and highly fatal septicaemia may cause high mortality. Therefore, early diagnosis and effective treatment may help to control the mortality. The present study was aimed to evaluate the suitability of various samples (blood, bone marrow, liver and lung) for isolation of Pasteurella multocida and their susceptibility against various antibiotics. Total 52 samples [blood (n= 25), bone marrow (n=9), liver (n=9) and pneumonic lung (n=9)] were collected from HS-suspected animals and subjected for bacterial isolation after confirming the disease using microscopy. Total 10 isolates [2 (blood), 2 (bone marrow), 1 (liver) and 5 (lung)] were isolated and confirmed by morphological characteristics, biochemical test and Polymerase Chain reaction (PCR). Routinely used antibiotics were screened for their sensitivity against these isolates. Based on the present study, it may be concluded that the lung tissue sample may be the ideal sample for P. multocida isolation, whereas, gentamicin and chloramphenicol are effective antibiotics against all the isolates.