Journal of Immunology and Immunopathology
  • Year: 2018
  • Volume: 20
  • Issue: 1

Cytological and Microbiological Evaluation of Nasal Lavage of Calves Naturally Infected with Bovine Respiratory Disease

  • Author:
  • A. G. Bhanuprakash1, Vivek Joshi1, V.K. Gupta1, O.R. Vinodh Kumar2,*, Aron Jacob1, N. Shruthi3, B.S. Pruthvishree4
  • Total Page Count: 4
  • Published Online: Jan 1, 2018
  • Page Number: 43 to 46

1Division of Medicine, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India

2Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India

3Division of Animal Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India

4Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India

*Corresponding author email id: vinodgupta1288@gmail.com

Abstract

A total of 12 calves with bovine respiratory disease (BRD), and 12 apparently healthy calves were selected based on clinical score system. The nasal lavage was collected for cytological analysis and bacterial isolation. Isolated bacteria [Staphylococcus aureus (58.33%), Escherichia coli (25%), Diplococci, Streptococci and E. coli (16.67%)] were characterised up to species level on the basis of standard biochemical tests. The differential cell cytology revealed a marked increase in the number of neutrophils and a significant decrease in lymphocytes and macrophages. An acute inflammatory response in calves during BRD may be responsible for changes in nasal lavage fluid. Coagulase positive S. aureus was the most predominant bacteria causing BRD in calves. Nasal cytology is an indicator of lower respiratory tract infections.

Keywords

Bovine respiratory disease, Calves, Cytology, Nasal lavage, Neutrophils