Updates in Diagnostic Techniques for Brucellosis in Livestock

Brucellosis is a zoonotic disease affecting human as well as domestic animals with worldwide distribution. There are several direct and indirect methods are available for diagnosis of brucellosis in animals. Traditionally, isolation of bacteria by culturing on selective media followed by bacteriological characterisation was done for diagnosis, but it is time consuming. Serological tests are having its own importance for laboratory diagnosis of brucellosis as most of the papers published till date use these methods for screening samples. With advancement in molecular biology and biotechnology, different techniques like polymerase chain reaction (PCR), real-time PCR, loop-mediated isothermal amplification assay are gaining importance for diagnosis of brucellosis in livestock now a day's. These methods are more sensitive and give more accurate results than traditional methods.