Identification of Invasive and Biofilm Producing Salmonella Species of Broiler from Tribal Area of Hoshangabad District, Madhya Pradesh

The present experiment was designed to identify the invasive and biofilm producing Salmonella organism of broilers from tribal area of Hoshagabad district, Madhya Pradesh. A total of 153 samples consisting of cloacal swab (99), dry faeces (18), feed (18) and water (18) were collected in buffered peptone water during rainy, winter and summer seasons. Selective enrichment was performed, in Rappaport-Vassiliadis broth followed by streaking on MacConkey agar, brilliant green agar, xylose-lysine deoxycholate media, for the isolation. Phenotypic identification was done by motility test and Gram's staining. Biochemical tests like oxidase, catalase, indole, methyl red, Voges-Proskauer, triple sugar iron agar, urease were used for presumptive confirmation, and they were confirmed by latex agglutination test. All the biochemically confirmed isolates (13 isolates) were further confirmed by polymerase chain reaction by using 16S rRNA genus-specific gene and invA gene. The percentage-wise isolation of Salmonella organism from broilers in all the three seasons were as cloacal swab 5.55% (rainy), 3.44% (winter) and 14.70% (summer), although from dry faeces it was 16.66% in rainy and summer season, and from feed samples was 16.66% in rainy season. Overall, the incidence of invasive salmonellosis in broilers from tribal area of Hoshangabad district was 8.49%. Invasiveness and biofilm formation of salmonella isolates were directly correlated and discussed.