Journal of Immunology and Immunopathology

The aim of the present study was to observe the in-vitro antimicrobial susceptibility of 46 isolates of Clostridium perfringens from raw meat of buffen, chevon, chicken and fish. The antimicrobial susceptibility of C. perfringens was carried out by disc diffusion method. Out of 46 isolates, 82.60% were found sensitive to ofloxacin and amoxicillin each, 65.21% to cefotaxime, 54.34% to chloramphenicol, and resistant to amikacin –89.13%, co-trimoxazole – 69.56%, lincomycin –67.39% and ceftazidime –63.04%. Sample-wise antimicrobial profile study revealed that ofloxacin was found sensitive to 92.85% isolates from buffen followed by fish –91.66% and chevon and chicken –70% each. Amoxicillin showed sensitivity to 91.66% isolates from fish and 90.0% to chicken isolates. Cefotaxime was sensitive to 70% and 64.28% isolates from chevon and buffen, respectively. Amikacin showed 100% resistant towards isolates from buffen, followed by chevon (92.85%) and chicken (80%). All the isolates displayed multiple drug resistance with 28 isolates displayed resistance to more than five antimicrobials. The mean multiple antibiotic resistance (MAR) index of C. perfringens was 0.38. The MAR index of C. perfringens to 10 antibiotics was above 0.2. This study depicts the growing resistance in zoonotic food borne pathogen, which is an alarming signal as the bacteria is causing wide range of disease in both humans and animals.