Expression of Amino-terminal Fragment of Clostridium chauvoei toxin A

Clostridium chauvoei, the causative of black quarter in ruminants, is an anaerobic spore forming organism, produces different types of exotoxins. Clostridium chauvoei toxin A (cctA) is an important toxin possessing hemolytic and cytotoxic property and also claimed to be a protective antigen. Characterization of the different regions of the toxin has not been carried out yet. Hence, the regions responsible for different activities of the toxin are not clear. As an initial step, amino-terminal region (34-75aa) of cctA gene was cloned and expressed in pRham-N-His-Sumo-Kan vector. Expressed protein was confirmed by SDS-PAGE analysis.