Journal of Immunology and Immunopathology
  • Year: 2019
  • Volume: 21
  • Issue: 1

Cloning and Expression of Carboxyl-terminal Region of cctA Gene of Clostridium chauvoei

  • Author:
  • M. Mashooq1, K. Karthik1, V.K. Nagaleekar1,*
  • Total Page Count: 3
  • Published Online: Jan 1, 2019
  • Page Number: 47 to 49

12Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

*Corresponding author email id: vkn111@gmail.com

Abstract

The carboxyl-terminal region of cctA gene of Clostridium chauvoei was cloned and expressed in prokaryotic expression system. Further, expressed recombinant carboxyl-terminal region of cctA protein was purified and the expression was confirmed by SDS-PAGE analysis.

Keywords

Clostridium chauvoei, CctA gene, Carboxy-terminal, Cloning, Expression, PCR, Purification, SDS-PAGE