1Assistant Professor, Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, GBPUA&T, Pantnagar, Uttarakhand, India
2Professor, Department of Veterinary Physiology and Biochemistry
3Professor, Department of Veterinary Pathology, College College of Veterinary and Animal Sciences, GBPUA&T, Pantnagar, Uttarakhand, India
The cow urine is one of the ingredients of ‘Panchagavya’ and reported to exhibit several therapeutic potentials. It has also been observed during investigations that the urine of Indian cows is highly effective against various ailments. Cow urine or ‘gau mutra’ has a unique place in Ayurveda and is suggested for improving general health. Captan is a commonly used fungicide belonging to the chemical group sulfenamide. There are reports indicating its inhibitory role in cellular DNA synthesis in herring sperm DNA and human cellular DNA, suggesting a possible mutagenic consequence. It has shown to exert immunotoxic effects. Keeping in view of the above, present study was planned to explore counteracting role of cow urine against captan induced immunotoxic effects in chicken lymphocytes culture system. Present paper reports ameliorating effects of cow urine distillate (CU) against captan induced immunotoxicity and oxidative stress in chicken lymphocytes culture employing lymphocyte prol iferation assay and nitric oxide (NO) estimation. captan treated cells displayed immunotoxic effects as observed by decrease in B and T cells proliferation. In case of combination treatments of captan and CU, there was increase in B and T cells proliferation as compared to only pesticide treated cells. Nitric oxide estimation revealed enhanced oxidative stress in captan treated cells in comparison to combination treated groups. Thus, it could be inferred from the present study that CU displayed counteracting effects against captan induced immunotoxicity and oxidative stress due to
Captan, Immunotoxicity, Cow urine, Oxidative stress, Chicken lymphocytes, Nitric oxide
