1Assistant Director, CCS National Institute of Animal Health, Baghpat-250609, Uttar Pradesh, India
2M.V.Sc. Student, Centre of Animal Disease Diagnosis and Research, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India
3Scientist, Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122,Uttar Pradesh, India
4Scientist, Centre of Animal Disease Diagnosis and Research, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India
5Principal Scientist; Centre of Animal Disease Diagnosis and Research, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India
*Corresponding author email id: sukdebnandi@yahoo.in
Online published on 9 March, 2022.
Canine parvoviral enteritis remains one of the dreaded enteric viral diseases of dogs. Rapid detection of the etiological agent, Canine parvovirus-2 (CPV-2) is required for managemental intervention and initiating therapeutic and control measures. The present study describes Loop Mediated Isothermal Amplification (LAMP) using loop primers, validating the assay on all the circulating CPV-2 antigenic types and testing in 120 suspected clinical samples. The novel LAMP assay was completed within 30-45 min at 64°C in a water bath. Analytical sensitivity of the LAMP assay was 100 times more than conventional PCR and was comparable to real-time PCR. LAMP was able to detect 62 positive samples while conventional PCR could detect only in 51 samples. The developed LAMP provides a simple, rapid, cost-effective, sensitive, specific method of detection of CPV-2 infection in dogs in clinical settings.
LAMP, Canine parvovirus (CPV), CPV antigenic types
